"The CliniMACS® plus Instrument is a software-controlled instrument that processes the HPC, Apheresis as part of the CliniMACS CD34 Reagent System. The CliniMACS® CD34 Reagent System is an in vitro medical device system used to select and enrich CD34+ cells from HPC, Apheresis while passively depleting other cells, such as CD3+ T cells, which cause graft versus host disease. The system is based on ""magnetically-activated cell sorting"" (MACS) employing antibodies conjugated to iron-containing particles that can be attracted to a magnetic field (referred to as “magnetic labeling”). Using the specificity of anti-CD34 antibody interaction with cell surface CD34 antigen found on hematopoietic progenitor cells, the system enriches CD34+ cells from HPC, Apheresis by passing the antibody-labeled cell suspension through a separation column with a strong magnetic gradient. The separation column retains the magnetically labeled CD34+ target cells while unlabeled cells flow through and are collected in the Negative Fraction Bag. Several automated washing steps are performed, disposing most of the liquid into the Buffer Waste Bag. The magnetically-selected CD34+ cells are released from the separation column when the magnet is disengaged, removing the magnetic field, and the target CD34+ cells are eluted into the Cell Collection Bag."
A mains electricity (AC-powered) device that is a component of a magnetic cell separation system designed to magnetically separate specific cell types or subpopulations from heterogeneous human cell preparations. It is a programmable device typically consisting of pumping/agitating mechanisms, a magnet, valves, bag hangers and a user display screen. It functions in combination with a magnetizing reagent, a buffered washing solution and a tubing set to separate the target cell population by retention in a magnetic field. The cells are typically used in regenerative medicine (e.g., cardiovascular, haematogenic) or disease treatment (e.g., oncology). This is a reusable device.
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