2. DOSAGE AND ADMINISTRATION
2.1. To open blister package, peel cover film back 4/5 of its length.
2.2. Prepare the blood bag following your institution's standard operating procedures.
2.3. Make a loose knot in the donor tubing approximately 10 cm or 4 inches from the needle unless alternate methods are used to seal the tubing at the end of collection.
2.4. Temporarily clamp donor tubing.
2.5. Suspend the collection bag as far as possible below the donor's arm.
2.6. Apply blood pressure cuff or tourniquet to donor's arm. Disinfect site of phlebotomy. If blood pressure cuff is used, inflate to approximately 60 mmHg.
2.7. Remove needle cover and perform phlebotomy.
2.7.1. CAUTION: Do not touch the needle after removing the needle cover.
2.8. Remove the temporary clamp on the donor tubing to permit blood flow into the collection bag.
2.9. Secure donor tubing to donor's arm.
2.10. Mix blood with anticoagulant in the collection bag and continue to mix at several intervals during collection and immediately after collection. If using an automated mixer, follow manufacturer's instructions.
2.11. Collect labeled volume of blood 450 mL (+/- 10%).
2.12. When the desired amount of blood has been collected, seal the tubing or tighten the loose knot (white knot) prepared in Step 2.3. Temporarily clamp between the knot and needle. Sever donor tubing between knot and clamp. Alternate methods may be used to seal tubing. Collect blood samples using careful attention to aseptic technique and following your institution's standard operating procedures.
2.12.1. CAUTION: Do not use a dielectric tube sealer to seal the tubing while the needle is connected to the donor's body unless it is approved for such a purpose.
2.13. Reapply clamp to donor tubing; release pressure on donor's arm and remove needle. If using a needle safety device, follow manufacturer's instructions.
2.13.1. CAUTION: Discard phlebotomy needle/donor tubing according to institutional procedures.
2.14. Immediately after collection, invert collection bag several times to assure blood and anticoagulant are well mixed.
2.15. Strip blood from donor tubing into collection bag, mix well, and allow tubing to refill; repeat once. Make an appropriate number of segments of anticoagulated blood for testing by sealing on or near X marks. Leave segments attached to Whole Blood unit.
2.16. The time between Whole Blood collection and component separation may vary depending on both the blood bag system and processing options selected. Follow your institution's standard operating procedures to prepare components.
2.16.1. If the Whole Blood is to be processed into room temperature components, maintain the blood at ambient temperature.
2.16.2. If the Whole Blood is to be processed into other components (including Plasma Frozen Within 24 Hours After Phlebotomy), Whole Blood must either be placed in storage at a temperature between 1-6oC within 8 hours of blood collection or cooled towards a temperature between 1-10oC (e.g. during transport) and then placed in storage at a temperature between 1-6oC within 8 hours of blood collection or cooled towards a temperature between 1-10oC (e.g. during transport) and then placed in storage at a temperature between 1-6oC upon arrival at the processing center.
2.17. Platelet Rich Plasma and Platelets should be separated from the Red Blood Cells within 8 hours of blood collection, if prepared.
2.18. Plasma intended for production of Fresh Frozen Plasma should be separated from the Red Blood Cells and placed in a freezer at -18oC or colder within 8 hours of blood collection.
2.19. Plasma intended for production of Plasma Frozen Within 24 Hours After Phlebotomy (PF24) should be placed in a freezer at -18oC or colder within 24 hours of blood collection.
2.20. For further preparation and processing of other plasma components, use standard processing and storage techniques following approved regulations and standards.
2.21. Select the appropriate spin condition and centrifuge Whole Blood unit to separate CPDA-1 Red Blood Cells from plasma or platelet rich plasma, as appropriate.
2.22. Break the CLIKTIP (inline closure device) of primary collection bag and transfer plasma into satellite bag, or transfer platelet rich plasma into XT-612 Platelet Bag. Clamp transfer tubing of satellite bag.
2.23. Seal tubing of primary bag in two places, cut between seals, and if applicable, separate from satellite bag(s).
2.24. Store CPDA-1 Red Blood Cells (or Whole Blood) between 1-6oC for up to 35 days.
2.25. If prepared, store Platelets between 20-24oC for up to 5 days in XT-612 bag.
